Sale!

Spliceosomal Pre mRNA Splicing Methods and Protocols 2014th Edition by Klemens Hertel 1627039796 9781627039796

Original price was: $50.00.Current price is: $35.00.

Spliceosomal Pre mRNA Splicing Methods and Protocols 1st Edition Klemens J. Hertel (Eds.) Digital Instant Download

Author(s): Klemens J. Hertel (eds.)
ISBN(s): 9781627039796, 1627039791
Edition: 1
File Details: PDF, 7.09 MB
Year: 2014
Language: english
SKU: EB-4659064 Category: Tag:

Spliceosomal Pre mRNA Splicing Methods and Protocols 2014th Edition by Klemens J. Hertel – Ebook PDF Instant Download/Delivery: 1627039796, 978-1627039796
Full dowload Spliceosomal Pre mRNA Splicing Methods and Protocols 2014th Edition after payment

 

Product details:

ISBN 10:         1627039796
ISBN 13: 978-1627039796
Author:          Klemens J. Hertel

Providing a guide to classical experimental approaches to decipher splicing mechanisms and experimental strategies that rely on novel multi-disciplinary approaches, Spliceosomal Pre-mRNA Splicing: Methods and Protocols describes the theory of alternative pre-mRNA splicing in seven introductory chapters and then introduces protocols and their theoretical background relevant for a variety of experimental research. These protocol chapters cover basic methods to detect splicing events, analyses of alternative pre-mRNA splicing in vitro and in vivo manipulation of splicing events and high-throughput and bioinformatic analyses of alternative splicing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and practical, Spliceosomal Pre-mRNA Splicing: Methods and Protocols will aid newcomers and seasoned molecular biologists in understanding the fascinating world of alternative splicing with the ultimate goal of paving the way for many new discoveries to come.

 

Spliceosomal Pre mRNA Splicing Methods and Protocols 2014th Table of contents:

Part I: Overview of Spliceosomal Pre-mRNA Splicing

  1. Introduction to Spliceosomal Pre-mRNA Splicing
    • The Spliceosome
    • Mechanisms of Splicing
    • Key Components of the Spliceosome
    • Spliceosomal Assembly and Catalysis

Part II: In Vitro Splicing Assays
2. In Vitro Splicing of Pre-mRNA Substrates

  • Protocol for Splicing Reaction
  • Isolating and Preparing Splicing Factors
  • Quantification of Splicing Efficiency
  1. Preparation of Spliceosomal Complexes for In Vitro Analysis
    • Purification of Spliceosomal Components
    • Reconstitution of Spliceosomes
    • Evaluation of Spliceosome Activity

Part III: In Vivo Approaches
4. Analysis of Splicing in Living Cells

  • Techniques for Monitoring Pre-mRNA Splicing
  • Live-Cell Imaging of Spliceosome Dynamics
  • Measuring Splicing Kinetics in Cells
  1. RNA Interference in Spliceosome Function
    • Targeting Splicing Factors Using siRNA
    • Knockdown Efficiency and Validation
    • Effects of Splicing Factor Knockdown on Pre-mRNA Splicing

Part IV: Structural and Functional Studies
6. Cryo-EM and X-ray Crystallography of the Spliceosome

  • Cryo-EM Protocols for Spliceosome Visualization
  • X-ray Crystallography of Splicing Complexes
  • Insights into Spliceosome Architecture
  1. Single-Molecule Approaches to Spliceosomal Dynamics
    • Fluorescence Microscopy and FRET
    • Single-Molecule RNA Binding Studies
    • Monitoring Spliceosome Activity at the Single-Molecule Level

Part V: Splicing and Disease
8. Splicing Mutations in Disease

  • Mutations in Splice Sites and Splicing Factors
  • Splicing Dysregulation in Cancer
  • Neurological Diseases and Spliceosome Defects
  1. Therapeutic Strategies Targeting Pre-mRNA Splicing
    • Small Molecule Modulators of Splicing
    • Antisense Oligonucleotides for Splicing Therapy
    • Targeted Therapy for Splicing-Related Diseases

Part VI: Experimental Tools and Technologies
10. Next-Generation Sequencing to Study Splicing
– RNA-Seq for Splicing Profiling
– Analysis of Alternative Splicing Events
– Bioinformatics Tools for Splicing Data Analysis
11. High-Throughput Screening for Splicing Modulators
– Designing Splicing-Related Screens
– Identifying Small Molecule Splicing Modulators
– In Vivo Screening Assays

Part VII: Conclusions and Future Directions
12. Emerging Technologies in Splicing Research
– Advances in Splicing Reporter Systems
– Novel Methods for Spliceosome Purification
– The Future of Splicing Research and Therapeutics

People also search for Spliceosomal Pre mRNA Splicing Methods and Protocols 2014th:

splicing mrna processing
pre-mrna splice sites are recognized by the spliceosome through
dna splicing process
gene splicing process
mrna splicing in eukaryotes

Tags:

Klemens Hertel,Spliceosomal,Pre mRNA,Splicing Methods,Protocols 2014th