Mouse Genetics Methods and Protocols 1st Edition by Shree Ram Singh, Vincenzo Coppola ISBN 978-1493945672 149394567X

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Product details: 

ISBN 10:  149394567X

ISBN 13: 978-1493945672

Author: Shree Ram Singh, Vincenzo Coppola

Mouse Genetics: Methods and Protocols provide selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplishthe manipulation of genes of interest, tracing cell lineages and modeling human diseases. Composed in the highly successful Methods in Molecular Biology series format, each chapter contains a brief introduction, a list of necessary materials, systematic, readily reproducible methods and a notes section, which shares tips on troubleshooting in order to avoid known pitfalls.

Comprehensive and authoritative, Mouse Genetics: Methods and Protocols promises to deliver fundamental techniques and protocols to geneticists, molecular biologists, cell and developmental biologists, students and postdoctoral fellows working in the various disciplines of mouse biology and modeling human disease.

Table of contents: 

1. Development of Pronuclear Injection-Based Targeted Transgenesis in Mice Through Cre–loxP Site-Specific Recombination

2. Generation of Conditional Knockout Mice

3. Generating Mouse Lines for Lineage Tracing and Knockout Studies

4. Generation of an Allelic Series of Knock-In Mice Using Recombinase-Mediated Cassette Exchange (RMCE)

5. Generating Chimeric Mice from Embryonic Stem Cells via Vial Coculturing or Hypertonic Microinjection

6. Genetic Inducible Fate Mapping in Adult Mice Using Tamoxifen-Dependent Cre Recombinases

7. Intracytoplasmic Sperm Injection (ICSI)-Mediated Transgenesis in Mice

8. Generation of Bacterial Artificial Chromosome (BAC) Transgenic Mice

9. Generation of Genetically Engineered Mice by the piggyBac Transposon System

10. Generation and Applications of MADM-Based Mouse Genetic Mosaic System

11. Generation of Mouse Lines Conditionally Over-Expressing MicroRNA Using the Rosa26-Lox-Stop-Lox System

12. In Situ Hybridization (Both Radioactive and Non-Radioactive) and Spatiotemporal Gene Expression Analysis

13. Isolation and Handling of Mouse Embryonic Fibroblasts

14. Generation of Induced Pluripotent Stem Cells from Mouse Adipose Tissue

15. Pdx1 (GFP/w) Mice for Isolation, Characterization and Differentiation of Pancreatic Progenitor Cells

16. Generation of Transgenic Mouse Fluorescent Reporter Lines for Studying Hematopoietic Development

17. Analyzing Gene Function in Adult Long-Term Hematopoietic Stem Cells Using the Interferon Inducible Mx1-Cre Mouse System

18. Generation of Transgenic Mice by Exploiting Spermatogonial Stem Cells In Vivo

19. Methods for the Detection of Genome Instability Derived from Replication Stress in Primary Mouse Embryonic Fibroblasts

20. Transgenic Nude Mice Ubiquitously-Expressing Fluorescent Proteins for Color-Coded Imaging of the Tumor Microenvironment

21. Genetically Engineered Insertional Mutagenesis in Mice to Model Cancer: Sleeping Beauty

22. Generating Double Knockout Mice to Model Genetic Intervention for Diabetic Cardiomyopathy in Humans

23. Experimental Osteoarthritis Models in Mice

24. Mouse Models and Methods for Studying Human Disease, Acute Kidney Injury (AKI)

25. Mouse Models of Acute and Chronic Colitis

26. Manipulation and Assessment of Gut Microbiome for Metabolic Studies

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Tags: Shree Ram Singh, Vincenzo Coppola, Mouse Genetics, Methods and Protocols