Plant Pathogen Interactions Methods and Protocols 2nd Edition by Paul Birch, John Jones, Jorunn Bos ISBN 9781627039857 1627039856

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Product details: 

ISBN 10:  1627039856 

ISBN 13:   9781627039857

Author:  Paul Birch, John T. Jones, Jorunn I.B. Bos

Plant-Pathogen Interactions: Methods and Protocols, Second Edition expands upon the first edition with current, detailed protocols for the study of plant pathogen genome sequences. It contains new chapters on techniques to help identify and characterize effectors and to study their impacts on host immunity and their roles in pathogen biology. Additional chapters focus on protocols to identify avirulence and resistance genes, investigate the roles of effector targets and other defence-associated proteins in plant immunity. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols and tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Plant-Pathogen Interactions: Methods and Protocols, Second Edition seeks to aid scientists in the further study of plant immunity.

Table of contents: 

• Chapter 1 – Galaxy as a Platform for Identifying Candidate Pathogen Effectors
  Authors: Peter J. A. Cock, Leighton Pritchard

• Chapter 2 – Bioinformatic Analysis of Expression Data to Identify Effector Candidates
  Authors: Adam J. Reid, John T. Jones

• Chapter 3 – Two-Dimensional Data Binning for the Analysis of Genome Architecture in Filamentous Plant Pathogens and Other Eukaryotes
  Authors: Diane G. O. Saunders, Joe Win, Sophien Kamoun, Sylvain Raffaele

• Chapter 4 – On the Statistics of Identifying Candidate Pathogen Effectors
  Authors: Leighton Pritchard, David Broadhurst

Cell Biology

• Front Matter

• Chapter 5 – High-Throughput Imaging of Plant Immune Responses
  Authors: Martina Beck, Ji Zhou, Christine Faulkner, Silke Robatzek

• Chapter 6 – In Vivo Protein–Protein Interaction Studies with BiFC: Conditions, Cautions, and Caveats
  Authors: Petra Boevink, Hazel McLellan, Tatyana Bukharova, Stefan Engelhardt, Paul Birch

• Chapter 7 – Particle Bombardment-Mediated Transient Expression to Identify Localization Signals in Plant Disease Resistance Proteins and Target Sites for the Proteolytic Activity of Pathogen Effectors
  Authors: Daigo Takemoto, David A. Jones

• Chapter 8 – Purification of Fungal Haustoria from Infected Plant Tissue by Flow Cytometry
  Authors: Diana P. Garnica, John P. Rathjen

From Assays of Pathogen Virulence to Effector Function

• Front Matter

• Chapter 9 – Functional Characterization of Nematode Effectors in Plants
  Authors: Axel A. Elling, John T. Jones

• Chapter 10 – Silencing of Aphid Genes by Feeding on Stable Transgenic Arabidopsis thaliana
  Authors: Alexander D. Coleman, Marco Pitino, Saskia A. Hogenhout

• Chapter 11 – Leaf-Disc Assay Based on Transient Over-Expression in Nicotiana benthamiana to Allow Functional Screening of Candidate Effectors from Aphids
  Authors: Patricia A. Rodriguez, Saskia A. Hogenhout, Jorunn I. B. Bos

• Chapter 12 – A Growth Quantification Assay for Hyaloperonospora arabidopsidis Isolates in Arabidopsis thaliana
  Authors: Daniel F. A. Tomé, Jens Steinbrenner, Jim L. Beynon

• Chapter 13 – Simple Quantification of In Planta Fungal Biomass
  Authors: Michael Ayliffe, Sambasivam K. Periyannan, Angela Feechan, Ian Dry, Ulrike Schumann, Evans Lagudah et al.

• Chapter 14 – Virus-Induced Gene Silencing and Agrobacterium tumefaciens-Mediated Transient Expression in Nicotiana tabacum
  Authors: Zhao Zhang, Bart P. H. J. Thomma

• Chapter 15 – DIGE-ABPP by Click Chemistry: Pairwise Comparison of Serine Hydrolase Activities from the Apoplast of Infected Plants
  Authors: Tram Ngoc Hong, Renier A. L. van der Hoorn

• Chapter 16 – A Simple and Fast Protocol for the Protein Complex Immunoprecipitation (Co-IP) of Effector: Host Protein Complexes
  Authors: Jens Steinbrenner, Matthew Eldridge, Daniel F. A. Tomé, Jim L. Beynon

• Chapter 17 – An Arabidopsis and Tomato Mesophyll Protoplast System for Fast Identification of Early MAMP-Triggered Immunity-Suppressing Effectors
  Authors: Malou Fraiture, Xiangzi Zheng, Frédéric Brunner

• Chapter 18 – Production of RXLR Effector Proteins for Structural Analysis by X-Ray Crystallography
  Authors: Richard K. Hughes, Mark J. Banfield

Methods to Identify Resistance Genes and Avirulence Genes

• Front Matter

• Chapter 19 – The Do’s and Don’ts of Effectoromics
  Authors: Juan Du, Vivianne G. A. A. Vleeshouwers

• Chapter 20 – Protoplast Cell Death Assay to Study Magnaporthe oryzae AVR Gene Function in Rice
  Authors: Hiroyuki Kanzaki, Kentaro Yoshida, Hiromasa Saitoh, Muluneh Tamiru, Ryohei Terauchi

• Chapter 21 – A Bacterial Type III Secretion-Based Delivery System for Functional Assays of Fungal Effectors in Cereals
  Authors: Narayana M. Upadhyaya, Jeffery G. Ellis, Peter N. Dodds

• Chapter 22 – Genomic DNA Library Preparation for Resistance Gene Enrichment and Sequencing (RenSeq) in Plants
  Authors: Florian Jupe, Xinwei Chen, Walter Verweij, Kamel Witek, Jonathan D. G. Jones, Ingo Hein

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Tags: Paul Birch, John Jones, Jorunn Bos, Plant Pathogen Interactions, Methods and Protocols