Mitochondrial Bioenergetics Methods and Protocols 1st Edition by Carlos Palmeira, António Moreno ISBN 978-1617793820 1617793820

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Product details: 

ISBN 10: 1617793820

ISBN 13: 978-1617793820

Author: Carlos M. Palmeira, António J. Moreno

Increasing interest in mitochondrial bioenergetics is being driven by the impact of drug and environmental chemical-induced disturbances of mitochondrial function as well as hereditary deficiencies and the progressive deterioration of bioenergetic performance with age.  These initiatives have fostered the investigation of genetic and environmental influences on bioenergetics. In Mitochondrial Bioenergetics: Methods and Protocols, researchers in the field detail the practical principles and assays designed to derive quantitative assessment of each set of parameters that reflect different aspects of mitochondrial bioenergetics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls

Authoritative and practical, Mitochondrial Bioenergetics: Methods and Protocols helps elevate the quality and rate of investigative discoveries regarding disease states associated with environmental or genetic influences on mitochondrial bioenergetics.

Table of contents:

1 Overview of Mitochondrial Bioenergetics
2 Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells
3 High-Resolution Respirometry: OXPHOS Protocols for Human Cells and Permeabilized Fibers from Small Biopsies of Human Muscle
4 High-Throughput Analysis of Mitochondrial Oxygen Consumption
5 Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices
6 Mitochondrial Membrane Potential (ΔΨ) Fluctuations Associated with the Metabolic States of Mitochondria
7 Safranine as a Fluorescent Probe for the Evaluation of Mitochondrial Membrane Potential in Isolated Organelles and Permeabilized Cells
8 Fluorescence Measurement of Mitochondrial Membrane Potential Changes in Cultured Cells
9 Phenomenological Kinetic and Control Analysis of Oxidative Phosphorylation in Isolated Mitochondria
10 Expression of Uncoupling Proteins in a Mammalian Cell Culture System (HEK293) and Assessment of Their Protein Function
11 Measurement of Proton Leak and Electron Leak in Isolated Mitochondria
12 Relation Between Mitochondrial Membrane Potential and ROS Formation
13 Use of a Calcium-Sensitive Electrode for Studies on Mitochondrial Calcium Transport
14 Imaging Mitochondrial Calcium Signalling with Fluorescent Probes and Single or Two Photon Confocal Microscopy
15 Mitochondrial Permeability Transition Pore and Calcium Handling
16 Imaging of Mitochondrial pH Using SNARF-1
17 Redox Equivalents and Mitochondrial Bioenergetics
18 NMR Methodologies for Studying Mitochondrial Bioenergetics
19 Computational Modeling of Mitochondrial Function 

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Tags: Carlos Palmeira, António Moreno, Mitochondrial Bioenergetics, Methods and Protocols