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PCR Protocols 2nd Edition by John Bartlett, David Stirling ISBN 0896036278 978-0896036277

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PCR Protocols 2nd Edition John M. S. Bartlett Digital Instant Download

Author(s): John M. S. Bartlett, David Stirling (auth.), John M. S. Bartlett, David Stirling (eds.)
ISBN(s): 9781592593842, 1592593844
Edition: 2
File Details: PDF, 14.42 MB
Year: 2003
Language: english
SKU: EB-917630 Category: Tags: , ,

PCR Protocols 2nd Edition by John M. S. Bartlett, David Stirling – Ebook PDF Instant Download/Delivery: 0896036278, 978-0896036277
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Product details: 

ISBN 10: 0896036278 

ISBN 13: 978-0896036277

Author: John M. S. Bartlett, David Stirling 

Drawing on the highly successful first edition, this newly-revised second edition covers the many advances made in PCR technology since the first book, which has been used in more than 10,000 laboratories worldwide. As PCR technology has advanced significantly since the first edition, and has expanded its use in the clinical laboratory of physician/researchers, the scope of this book is greatly expanded to enable researchers at all levels to easily reproduce and adapt PCR experiments to their own specific requirements. The meethods selected represent worked examples from many fields that can be reproduced and adapted for use within the reader’s laboratory. The authors have provided both a primer to allow the reader to gain basic experience of different PCR techniques, as well as in-depth insight into a variety of the more complex applications of PCR. This book will be essential for the labs of all biochemists, molecular biologists, geneticists and researchers utilizing the PCR techinque in their work.
 

Table of contents: 

  1. A Short History of the Polymerase Chain Reaction

  2. PCR Patent Issues

  3. Equipping and Establishing a PCR Laboratory

  4. Quality Control in PCR

  5. Extraction of Nucleic Acid Templates

  6. Extraction of DNA from Whole Blood

  7. DNA Extraction from Tissue

  8. Extraction of DNA from Microdissected Archival Tissues

  9. RNA Extraction from Blood

  10. RNA Extraction from Frozen Tissue

  11. RNA Extraction from Tissue Sections

  12. Dual DNA/RNA Extraction

  13. DNA Extraction from Fungi, Yeast, and Bacteria

  14. Isolation of RNA Viruses from Biological Materials

  15. Extraction of Ancient DNA

  16. DNA Extraction from Plasma and Serum

  17. Technical Notes for the Detection of Nucleic Acids

  18. Technical Notes for the Recovery and Purification of PCR Products from Acrylamide Gels

  19. PCR Primer Design

  20. Optimization of Polymerase Chain Reactions

  21. Subcycling PCR for Long-Distance Amplifications of Regions with High and Low Guanine-Cystine Content: Amplification of the Intron 22 Inversion of the FVIII Gene

  22. Rapid Amplification of cDNA Ends

  23. Randomly Amplified Polymorphic DNA Fingerprinting: The Basics

  24. Microsphere-Based Single Nucleotide Polymorphism Genotyping

  25. Ligase Chain Reaction

  26. Nested RT-PCR in a Single Closed Tube

  27. Direct PCR from Serum: Application to Viral Genome Detection

  28. Long PCR Amplification of Large Fragments of Viral Genomes: A Technical Overview

  29. Long PCR Methodology

  30. Qualitative and Quantitative PCR: A Technical Overview

  31. Ultrasensitive PCR Detection of Tumor Cells in Myeloma

  32. Ultrasensitive Quantitative PCR to Detect RNA Viruses

  33. Quantitative PCR for cAMP RI Alpha mRNA: Use of Site-Directed Mutation and PCR Mimics

  34. Quantitation of Multiple RNA Species

  35. Differential Display: A Technical Overview

  36. AU-Differential Display, Reproducibility of a Differential mRNA Display Targeted to AU Motifs

  37. PCR Fluorescence Differential Display

  38. Microarray Analysis Using RNA Arbitrarily Primed PCR

  39. Oligonucleotide Arrays for Genotyping: Enzymatic Methods for Typing Single Nucleotide Polymorphisms and Short Tandem Repeats

  40. Serial Analysis of Gene Expression

  41. Mutation and Polymorphism Detection: A Technical Overview

  42. Combining Multiplex and Touchdown PCR for Microsatellite Analysis

  43. Detection of Microsatellite Instability and Loss of Heterozygosity Using DNA Extracted from Formalin-Fixed Paraffin-Embedded Tumor Material by Fluorescence-Based Multiplex Microsatellite PCR

  44. Reaction of Shadow Band Synthesis During PCR Amplification of Repetitive Sequences from Modern and Ancient DNA

  45. Degenerate Oligonucleotide-Primed PCR

  46. Mutation Detection Using RT-PCR-RFLP

  47. Multiplex Amplification Refractory Mutation System for the Detection of Prothrombotic Polymorphisms

  48. PCR-SSCP Analysis of Polymorphism: A Simple and Sensitive Method for Detecting Differences Between Short Segments of DNA

  49. Sequencing: A Technical Overview

  50. Preparation and Direct Automated Cycle Sequencing of PCR Products

  51. Nonradioactive PCR Sequencing Using Digoxigenin

  52. Direct Sequencing by Thermal Asymmetric PCR

  53. Analysis of Nucleotide Sequence Variations by Solid-Phase Minisequencing

  54. Direct Sequencing with Highly Degenerate and Inosine-Containing Primers

  55. Determination of Unknown Genomic Sequences Without Cloning

  56. Cloning PCR Products for Sequencing in M13 Vectors

  57. DNA Rescue by the Vectorette Method

  58. Technical Notes for Sequencing Difficult Templates

  59. PCR-Based Detection of Nucleic Acids in Chromosomes, Cells, and Tissues: Technical Considerations on PRINS and In Situ PCR, and Comparison with In Situ Hybridization

  60. Cycling Primed In Situ Amplification

  61. Direct and Indirect In Situ PCR

  62. Reverse Transcriptase In Situ PCR: New Methods in Cellular Interrogation

  63. Primed In Situ Nucleic Acid Labeling Combined with Immunocyhemistry to Simultaneously Localize DNA and Proteins in Cells and Chromosomes

  64. Cloning and Mutagenesis: A Technical Overview

  65. Using T4 DNA Polymerase to Generate Clonable PCR Products

  66. A T-Linker Strategy for Modification and Directional Cloning of PCR Products

  67. Cloning Gene Family Members Using PCR with Degenerate Oligonucleotide Primers

  68. cDNA Libraries from a Low Amount of Cells

  69. Creation of Chimeric Junctions, Deletions, and Insertions by PCR

  70. Recombination and Site-Directed Mutagenesis Using Recombination PCR

  71. Megaprimer PCR: Application in Mutagenesis and Gene Fusio

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