Protein Ligand Interactions Methods and Applications 2nd Edition by Mark Williams, Tina Daviter ISBN 978-1627033978 1627033971

Protein Ligand Interactions Methods and Applications 2nd Edition by Mark A. Williams, Tina Daviter – Ebook PDF Instant Download/Delivery: 978-1627033978, 1627033971
Full download Protein Ligand Interactions Methods and Applications 2nd Edition after payment

Product details: 

ISBN 10: 1627033971

ISBN 13: 978-1627033978

Author: Mark A. Williams, Tina Daviter

Proteins are the cell’s workers, their messengers and overseers. In these roles, proteins specifically bind small molecules, nucleic acid and other protein partners. Cellular systems are closely regulated and biologically significant changes in populations of particular protein complexes correspond to very small variations of their thermodynamics or kinetics of reaction. Interfering with the interactions of proteins is the dominant strategy in the development of new pharmaceuticals. Protein Ligand Interactions: Methods and Applications, Second Edition provides a complete introduction to common and emerging procedures for characterizing the interactions of individual proteins. From the initial discovery of natural substrates or potential drug leads, to the detailed quantitative understanding of the mechanism of interaction, all stages of the research process are covered with a focus on those techniques that are, or are anticipated to become, widely accessible and performable with mainstream commercial instrumentation. Written in the highly successful Methods in Molecular Biology series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and accessible, Protein Ligand Interactions: Methods and Applications, Second Edition serves as an ideal guide for researchers new to the field of biophysical characterization of protein interactions – whether they are beginning graduate students or experts in allied areas of molecular cell biology, microbiology, pharmacology, medicinal chemistry or structural biology.

Table of contents: 

1 Protein-Ligand Interactions: Fundamentals
2 Protein Sample Characterization
3 Measurement of Protein-Ligand Complex Formation
4 Isothermal Titration Calorimetry for Studying Protein-Ligand Interactions
5 Rapid Mixing Kinetic Techniques
6 Protein-Ligand Interactions Using SPR Systems
7 Fluorescence Techniques in Analysis of Protein-Ligand Interactions
8 Circular and Linear Dichroism Spectroscopy for the Study of Protein-Ligand Interactions
9 Analyzing Protein-Ligand Interactions by Dynamic NMR Spectroscopy
10 Studying Metal Ion – Protein Interactions: Electronic Absorption, Circular Dichroism and Electron Paramagnetic Resonance
11 Monitoring Protein-Ligand Interactions by Time-resolved FTIR Difference Spectroscopy
12 Biophysical Methods in Drug Discovery from Small Molecule to Pharmaceutical
13 Biophysical Screening for the Discovery of Small-Molecule Ligands
14 Screening Protein-Small Molecule Interactions by NMR
15 Model Membrane Systems
16 Quantitative Fluorescence Co-localization to Study Protein-Receptor Complexes
17 Studying Protein-Ligand Interactions Using X-Ray Crystallography
18 Molecular Fields in Ligand Discovery
19 Structure-Based Virtual Screening for Novel Ligands

People also search for:

protein ligand interactions methods
    
protein ligand interactions methods and applications
    
insights into protein ligand interactions mechanisms models and methods
    
nmr methods to characterize protein ligand interactions
    
what is protein ligand interaction

Tags: Mark Williams, Tina Daviter, Protein Ligand, Interactions Methods