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Metagenomics Methods and Protocols 3rd Edition by Wolfgang Streit, Rolf Daniel 1071627945 9781071627945

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Metagenomics Methods and Protocols 3rd Edition Wolfgang R. Streit Digital Instant Download

Author(s): Wolfgang R. Streit, Rolf Daniel
ISBN(s): 9781493982745, 1493982745
Edition: 3
File Details: PDF, 8.74 MB
Year: 2022
Language: english
SKU: EB-47214044 Category: Tags: ,

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Product details:

ISBN 10:         1071627945
ISBN 13: 978-1071627945
Author:          Wolfgang R. Streit, Rolf Daniel

This volume provides up-to-date and novel techniques for various screening technologies currently used in metagenomics and related areas. Starting with DNA/RNA isolation from environmental samples, the book continues by delving into areas such as current methods used to isolate DNA and construct metagenomic libraries, establishment of metagenome libraries in non-E. coli hosts, and topics like function-driven mining of metagenomic DNA, screening and analyzing protocols for a wide array of different genes encoding enzymes, bacterial viruses and much more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Metagenomics: Methods and Protocols, Third Edition provides a comprehensive collection of up-to-date metagenome protocols and tools for the recovery of many major types of biocatalysts and allows for the easy setup of these screens in microbiology laboratories.
 

Metagenomics Methods and Protocols 3rd Table of contents:

    1. Introduction

      • Overview of Molecular Methods for Studying Complex Microbial Communities

    2. Construction of Small-Insert and Large-Insert Metagenomic Libraries

      • Techniques and Approaches for Library Construction
      • Advantages and Challenges of Different Inserts

    3. Construction and Screening of Marine Metagenomic Libraries

      • Marine Microbial Diversity and Its Relevance to Metagenomics
      • Steps in the Library Construction and Screening Process

    4. Metagenomic Analysis of Isotopically Enriched DNA

      • Isotopic Labeling Methods
      • Insights Gained from Isotopically Enriched DNA

    5. Wide Host-Range Cloning for Functional Metagenomics

      • Importance of Host Range in Cloning
      • Approaches to Expanding Host-Range Cloning Systems

    6. Cloning and Expression Vectors for a Gram-Positive Host, Streptomyces lividans

      • Overview of Streptomyces lividans as a Host System
      • Cloning and Expression Strategies

    7. Retracted Chapter: Heterologous Gene Expression in the Hyperthermophilic Archaeon Sulfolobus solfataricus

      • Erratum and Details of the Retraction

    8. Novel Tools for the Functional Expression of Metagenomic DNA

      • Innovative Techniques for Functional Expression
      • Applications in Metagenomics

    9. Screening of Functional Promoters from Metagenomic DNA for Practical Use in Expression Systems

      • Methods for Identifying Active Promoters
      • Practical Applications of Promoter Screening

    10. Substrate-Induced Gene Expression Screening: A Method for High-Throughput Screening of Metagenome Libraries

    • Overview of Substrate-Induced Screening
    • High-Throughput Screening Methods and Applications
    1. Screens for Active and Stereoselective Hydrolytic Enzymes
    • Screening Approaches for Hydrolytic Enzymes
    • Focus on Stereoselectivity
    1. Screening for Cellulase-Encoding Clones in Metagenomic Libraries
    • Techniques for Identifying Cellulase-Encoding Genes
    • Importance for Industrial and Environmental Applications
    1. Screening Metagenomic Libraries for Laccase Activities
    • Laccases and Their Importance in Biotechnology
    • Methods for Screening Laccase-Encoding Genes
    1. Screening for N-AHSL-Based-Signaling Interfering Enzymes
    • Quorum Sensing and N-AHSL Signaling
    • Screening for Enzymes that Interfere with Signaling
    1. Identification of Molecular Markers to Follow Up the Bioremediation of Sites Contaminated with Chlorinated Compounds
    • Role of Metagenomics in Bioremediation
    • Molecular Markers for Chlorinated Compound Degradation
    1. Methods for the Isolation of Genes Encoding Novel PHB Cycle Enzymes from Complex Microbial Communities
    • Biochemical Role of PHB Cycle Enzymes
    • Approaches for Isolating Relevant Genes
    1. Metagenomic Approaches to Identify and Isolate Bioactive Natural Products from Microbiota of Marine Sponges
    • Marine Sponges as a Source of Bioactive Compounds
    • Metagenomics for Natural Product Discovery
    1. Screening for Novel Antibiotic Resistance Genes
    • Methods for Identifying Antibiotic Resistance in Metagenomes
    • Implications for Public Health
    1. Novel Metal Resistance Genes from Microorganisms: A Functional Metagenomic Approach
    • Metal Resistance in Microbial Communities
    • Functional Metagenomic Screening for Resistance Genes
    1. Retrieval of Full-Length Functional Genes Using Subtractive Hybridization Magnetic Bead Capture
    • Magnetic Bead Capture for Gene Retrieval
    • Subtractive Hybridization Techniques
    1. Detection and Isolation of Selected Genes of Interest from Metagenomic Libraries by a DNA Microarray Approach
    • DNA Microarrays for Target Gene Detection
    • Applications in Metagenomic Library Screening
    1. Application of DNA Microarray for Screening Metagenome Library Clones
    • Protocols and Applications for DNA Microarray Screening
    • Case Studies and Results
    1. MetaGenomeThreader: A Software Tool for Predicting Genes in DNA-Sequences of Metagenome Projects
    • Overview of MetaGenomeThreader Software
    • Applications and Case Studies
    1. Erratum to: Heterologous Gene Expression in the Hyperthermophilic Archaeon Sulfolobus solfataricus
    • Correction and Additional Information on the Retraction

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