Plant Fungal Pathogens Methods and Protocols Methods in Molecular Biology Vol 835 2012th Edition by Melvin Bolton, Bart Thomma ISBN 9781617795008 1617795008

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ISBN 10:   1617795008

ISBN 13: 9781617795008

Author: Melvin D. Bolton, Bart P.H.J. Thomma

Over the course of evolution, fungi have adapted to occupy specific niches, from symbiotically inhabiting the flora of the intestinal tract of mammals to saprophytic growth on leaf litter resting on the forest floor.  In Plant Fungal Pathogens: Methods and Protocols, expert researchers in the field detail many of the methods which are now commonly used to study fungal plant pathogens. These include methods and techniques for model systems such as Arabidopsis thaliana as well as crop plants, aspects of fungal biology, genome annotation, next-generation sequencing, and fungal transformation and molecular tools for disease and/or pathogen quantification that are critical for revealing the role for a fungal gene of interest in disease development. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Table of contents: 

1 Fungal ABC Transporter Deletion and Localization Analysis. Andriy Kovalchuk, Stefan S. Weber, Jeroen G. Nijland, Roel A.L. Bovenberg, and Arnold J.M. Driessen

2 Targeted Gene Replacement in Fungal Pathogens via Agrobacterium tumefaciens-Mediated Transformation Rasmus John Normand Frandsen, Mette Frandsen, and Henriette Giese

3 Activity-Based Protein Profiling of Infected Plants. Farnusch Kaschani, Christian Gu, and Renier A.L. van der Hoorn

4 The Use of Agroinfiltration for Transient Expression of Plant Resistance and Fungal Effector Proteins in Nicotiana benthamiana Leaves. Lisong Ma, Ewa Lukasik, Fleur Gawehns, and Frank L.W. Takken

5 Proteomic Techniques for Plant-Fungal Interactions. Delphine Vincent, Kar-Chun Tan, Liam Cassidy, Peter S. Solomon, and Richard P. Oliver

6 Identification of HR-Inducing cDNAs from Plant Pathogens via a Gateway-Compatible Binary Potato Virus X-Expression Vector H. Peter van Esse

7 Freeze-Fracture of Infected Plant Leaves in Ethanol for Scanning Electron Microscopic Study of Fungal Pathogens Jayma A. Moore and Scott A. Payne

8 Real-Time PCR for the Quantification of Fungi In Planta. Steven J. Klosterman

9 Using Non-homologous End-Joining-Deficient Strains for Functional Gene Analyses in Filamentous Fungi. Mark Arentshorst, Arthur F.J. Ram, and Vera Meyer

10 Atomic Force Microscopy: A Tool for Studying Biophysical Surface Properties Underpinning Fungal Interactions with Plants and Substrates. Elizabeth Adams, Danielle Emerson, Sean Croker, Hye-Seon Kim, Shannon Modla, Seogchan Kang, and Kirk Czymmek

11 Use of the Yeast Two-Hybrid System to Identify Targets of Fungal Effectors Shunwen Lu

12 Characterization of Plant-Fungal Interactions Involving Necrotrophic Effector-Producing Plant Pathogens Timothy L. Friesen and Justin D. Faris

13 Heterologous Production of Fungal Effectors in Pichia pastoris. Anja Kombrink

14 The Application of Laser Microdissection to Profiling Fungal Pathogen Gene Expression in planta. Wei-Hua Tang, Yan Zhang, and Jon Duvick

15 Metabolomics Protocols for Filamentous Fungi. Joel P.A. Gummer, Christian Krill, Lauren Du Fall, Ormonde D.C. Waters, Robert D. Trengove, Richard P. Oliver, and Peter S. Solomon

16 Targeted Gene Replacement in Fungi Using a Split-Marker Approach….. Rubella S. Goswami

17 Massively Parallel Sequencing Technology in Pathogenic Microbes Sucheta Tripathy and Rays H.Y. Jiang

18 Confocal Microscopy in Plant-Pathogen Interactions Adrienne R. Hardham

19 The Use of Open Source Bioinformatics Tools to Dissect Transcriptomic Data. Benjamin M. Nitsche, Arthur F.J. Ram, and Vera Meyer

20 Population Biology of Fungal Plant Pathogens Zabi K. Atallah and Krishna V. Subbarao

21 Polyethylene Glycol (PEG)-Mediated Transformation in Filamentous Fungal Pathogens Zhaohui Liu and Timothy L. Friesen

22 In Vitro Induction of Infection-Related Hyphal Structures in Plant Pathogenic Fungi.. W.R. Rittenour and S.D. Harris

23 Fungicide Resistance Assays for Fungal Plant Pathogens Gary A. Secor and Viviana V. Rivera

24 Identification of Lipid-Binding Effectors. Shiv D. Kale and Brett M. Tyler

25 In Silico Identification and Characterization of Effector Catalogs… Ronnie de Jonge

26 Horizontal Transfer of Supernumerary Chromosomes in Fungi. H. Charlotte van der Does and Martijn Rep

27 The Induction of Mycotoxins by Trichothecene Producing Fusarium Species Rohan Lowe, Mélanie Jubault, Gail Canning, Martin Urban, and Kim E. Hammond-Kosack

28 Identifying Genes in Fusarium verticillioides Through Forward and Reverse Genetics J.B. Ridenour, R.L. Hirsch, and B.H. Bluhm

Contents

29 Assessment of Autophagosome Formation by Transmission Electron Microscopy. Marina Nadal and Scott E. Gold

30 Fungal Plant Pathogen Detection in Plant and Soil Samples Using DNA Macroarrays. B. Lievens, A. Justé, and K.A. Willems

31 Random Insertional Mutagenesis in Fungal Genomes to Identify Virulence Factors Parthasarathy Santhanam

32 A Yeast Secretion Trap Assay for Identification of Secreted Proteins from Eukaryotic Phytopathogens and Their Plant Hosts. Sang-Jik Lee and Jocelyn K.C. Rose

33 Comparing Fungal Genomes: Insight into Functional and Evolutionary Processes. Eva H. Stukenbrock and Julien Y. Dutheil

34 Multigene Phylogenetic Analyses to Delimit New Species in Fungal Plant Pathogens. Tara L. Rintoul, Quinn A. Eggertson, and C. André Lévesque

35 MAP Kinase Phosphorylation and CAMP Assessment in Fungi. Rahim Mehrabi, Sarrah Ben M’Barek, Abbas Saidi, Masoud Abrinbana, Pierre J.G.M. de Wit, and Gert H.J. Kema

36 A One-Step Affinity-Purification Protocol to Purify NB-LRR Immune Receptors from Plants That Mediate Resistance to Fungal Pathogens… Wladimir I.L. Tameling

37 Karyotyping Methods for Fungi Rahim Mehrabi, Masatoki Taga, Mostafa Aghace, Pierre J.G.M. de Wit, and Gert H.J. Kema

38 Isolation of Apoplastic Fluid from Leaf Tissue by the Vacuum Infiltration-Centrifugation Technique.

Matthieu H.A.J. Joosten

39 Gene Cloning Using Degenerate Primers and Genome Walking Javier A. Delgado, Steven Meinhardt, Samuel G. Markell, and Rubella S. Goswami

40 Construction of Hairpin RNA-Expressing Vectors for RNA-Mediated Gene Silencing in Fungi Shaobin Zhong, Yueqiang Leng, and Melvin D. Bolton

41 An Unbiased Method for the Quantitation of Disease Phenotypes Using a Custom-Built Macro Plugin for the Program ImageJ… Ahmed Abd-El-Haliem

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Tags: Melvin Bolton, Bart Thomma, Plant Fungal, Pathogens Methods