2D PAGE Sample Preparation and Fractionation 1st Edition by Barbara Sitek, Bence Sipos, Günter Klöppel ISBN 9781603272094 1603272097

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Product details: 

ISBN 10:  1603272097

ISBN 13: 9781603272094

Author: Barbara Sitek, Bence Sipos, Günter Klöppel
This volume provides an extensive exploration of sample preparation techniques essential for successful 2-DE analysis. It covers mechanical and enzymatic methods for cell disruption, solubilization buffers tailored for 2-DE, and strategies for protein quantification. The book also delves into the removal of interfering substances and discusses protein concentration methods, ensuring that researchers can achieve reproducible and high-quality results in their proteomic studies

Table of contents: 

1. Application of Fluorescence Dye Saturation Labeling for Differential Proteome Analysis of 1,000 Microdissected Cells from Pancreatic Ductal Adenocarcinoma Precursor Lesions

Barbara Sitek, Bence Sipos, Günter Klöppel, Wolff Schmiegel, Stephan A. Hahn, Helmut E. Meyer, and Kai Stühler

2. Albumin and Immunoglobulin Depletion of Human Plasma

Rosalind E. Jenkins, Neil R. Kitteringham, Carrie Greenough, and B. Kevin Park

3. Multi-Component Immunoaffinity Subtraction and Reversed-Phase Chromatography of Human Serum.

James Martosella and Nina Zolotarjova

4. Immunoaffinity Fractionation of Plasma Proteins by Chicken IgY Antibodies.

Lei Huang and Xiangming Fang

5. Proteomics of Cerebrospinal Fluid:

Methods for Sample Processing

John E. Hale, Valentina Gelfanova, Jin-Sam You, Michael D. Knierman, and Robert A. Dean

6. Sample Preparation of Bronchoalveolar Lavage Fluid. Baptiste Leroy, Paul Falmagne, and Ruddy Wattiez

7. Preparation of Nasal Secretions for Proteome Analysis. Begona Casado, Paolo ladarola, and Lewis K. Pannell

8. Preparation of Urine Samples for Proteomic Analysis. Rembert Pieper

9. Isolation of Cytoplasmatic Proteins from Cultured Cells for Two-Dimensional Gel Electrophoresis… Ying Wang, Jen-Fu Chiu, and Qing-Yu He

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Tags: Barbara Sitek, Bence Sipos, Günter Klöppel, 2D PAGE, Preparation and Fractionation