Transgenic Plants Methods and Protocols 2nd Edition by Jim Dunwell, Andy Wetten ISBN 9781617795572 1617795577

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Product details: 

ISBN 10:  1617795577

ISBN 13: 9781617795572

Author:  Jim M. Dunwell, Andy C. Wetten

In 2010 the global area of transgenic crops reached 148 million hectares, an 87-fold increase since 1996, making it the most rapidly adopted technology in the history of modern agriculture. In Transgenic Plants: Methods and Protocols, Second Edition expert researchers in the field provide key techniques to investigate production and analysis of transgenic plants.  Focusing on selection and detection methods, transformation technology, gene targeting, silencing and directed mutation, metabolic engineering and pharming, the book encompasses protocols relating to major crops and model plants being used for genomic analysis. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

Thorough and intuitive, Transgenic Plants: Methods and Protocols, Second Edition aids scientists in the continuous improvements being made for the production and analysis of transgenic plants.

Table of contents: 

1. SELECTION AND DETECTION METHODS
2. Employment of Cytokinin Vectors for Marker-Free and Backbone-Free Transformation
   Craig M. Richael, Caius M. Rommens
3. Organophosphorus Hydrolase: A Multifaceted Plant Genetic Marker Which Is Selectable, Scorable, and Quantifiable in Whole Seed
   T. Scott Pinkerton, James R. Wild, John A. Howard
4. Use of Northern Blotting for Specific Detection of Small RNA Molecules in Transgenic Plants
   Basel Khraiwesh
5. TRANSFORMATION TECHNOLOGY – RICE
6. A High-Efficiency Agrobacterium-Mediated Transformation System of Rice (Oryza sativa L.)
   Kenjirou Ozawa
7. Selection of Transgenic Rice Plants Using a Herbicide Tolerant Form of the Acetolactate Synthase Gene
   Masaki Endo, Tsutomu Shimizu, Seiichi Toki
8. Visual Selection in Rice: A Strategy for the Efficient Identification of Transgenic Calli Accumulating Transgene Products
   Hiroaki Saika, Haruko Onodera, Seiichi Toki
9. Characterization of Rice Genes Using a Heterologous Full-Length cDNA Expression System
   Mieko Higuchi, Youichi Kondou, Masaki Mori, Takanari Ichikawa, Minami Matsui
10. Bioactive Bead-Mediated Transformation of Plants with Large DNA Fragments
    Naoki Wada, Joyce A. Cartagena, Naruemon Khemkladngoen, Kiichi Fukui

1. TRANSFORMATION TECHNOLOGY – CEREALS AND OTHER MONOCOTS
2. Agrobacterium-Mediated Transformation of Sorghum bicolor Using Immature Embryos
   Songul Gurel, Ekrem Gurel, Tamara I. Miller, Peggy G. Lemaux
3. Split-Transgene Expression in Wheat
   Mario Gils, Myroslava Rubtsova, Katja Kempe
4. Agrobacterium-Mediated Transformation of Brachypodium distachyon
   Vera Thole, Philippe Vain
5. Transformation of Barley (Hordeum vulgare L.) by Agrobacterium tumefaciens Infection of In Vitro Cultured Ovules
   Inger Bæksted Holme, Henrik Brinch-Pedersen, Mette Lange, Preben Bach Holm
6. Biolistic-Mediated Production of Transgenic Oil Palm
   Ghulam Kadir Ahmad Parveez, Bohari Bahariah
7. Transformation of Oil Palm Using Agrobacterium tumefaciens
8. TRANSFORMATION TECHNOLOGY – DICOTS
9. Highly Efficient Transformation Protocol for Plum (Prunus domestica L.)
   César Petri, Ralph Scorza, Chinnathambi Srinivasan
10. Co-transformation of Grapevine Somatic Embryos to Produce Transgenic Plants Free of Marker Genes
    Manjul Dutt, Zhijian T. Li, Sadanand A. Dhekney, Dennis J. Gray
11. Initiation and Transformation of Grapevine Embryogenic Cultures
    Sadanand A. Dhekney, Zhijian T. Li, Manjul Dutt, Dennis J. Gray
12. Development of Highly Efficient Genetic Transformation Protocols for Table Grape Sugraone and Crimson Seedless
    Mercedes Dabauza, Leonardo Velasco
13. Cotton Pistil Drip Transformation Method
    Tianzhen Zhang, Tianzhi Chen
14. Enhanced Agrobacterium-Mediated Transformation of Embryogenic Calli of Upland Cotton
    Tianzhen Zhang, Shen-jie Wu
15. Targeted Biolistics for Improved Transformation of Impatiens balsamina
    Andy C. Wetten, Jean-Luc Thomas, Alina Wagiran, Tinashe Chiurugwi
16. A Protocol for Transformation of Torenia
    Ryutaro Aida
17. Efficient Modification of Floral Traits by Heavy-Ion Beam Irradiation on Transgenic Torenia
    Norihiro Ohtsubo, Katsutomo Sasaki, Ryutaro Aida, Hiromichi Ryuto, Hiroyuki Ichida, Yoriko Hayashi et al.

1. GENE TARGETING, SILENCING AND DIRECTED MUTATION
2. Expression of Artificial MicroRNAs in Physcomitrella patens
   Isam Fattash, Basel Khraiwesh, M. Asif Arif, Wolfgang Frank
3. High Frequency of Single-Copy T-DNA Transformants Produced After Floral Dip in CRE-Expressing Arabidopsis Plants
   Annelies De Paepe, Sylvie De Buck, Jonah Nolf, Ann Depicker
4. A Developmentally Regulated Cre-lox System to Generate Marker-Free Transgenic Brassica napus Plants
   Lilya Kopertekh, Inge Broer, Joachim Schiemann
5. Exploiting MultiSite Gateway and pENFRUIT Plasmid Collection for Fruit Genetic Engineering
   Leandro H. Estornell, Antonio Granell, Diego Orzaez
6. A One-Time Inducible Transposon to Create Knockout Mutants in Rice
   Yuh-Chyang Charng
7. Marker-Free Gene Targeting by Recombinase-Mediated Cassette Exchange
   Hiroyasu Ebinuma, Kazuya Nanto, Saori Kasahara, Atsushi Komamine
8. Targeting DNA to a Previously Integrated Transgenic Locus Using Zinc Finger Nucleases
   Tonya L. Strange, Joseph F. Petolino
9. Double-Strand Break-Induced Targeted Mutagenesis in Plants
   L. Alexander Lyznik, Vesna Djukanovic, Meizhu Yang, Spencer Jones
10. METABOLIC ENGINEERING AND PHARMING
11. Combinatorial Genetic Transformation of Cereals and the Creation of Metabolic Libraries for the Carotenoid Pathway
    Gemma Farre, Shaista Naqvi, Georgina Sanahuja, Chao Bai, Uxue Zorrilla-López, Sol M. Rivera et al.
12. Production of a His-Tagged Canecystatin in Transgenic Sugarcane
    Flavio Henrique-Silva, Andrea Soares-Costa
13. Plastid Transformation as an Expression Tool for Plant-Derived Biopharmaceuticals
    Nunzia Scotti, Teodoro Cardi
14. Use of a Callus-Specific Selection System to Develop Transgenic Rice Seed Accumulating a High Level of Recombinant Protein
    Yuhya Wakasa, Fumio Takaiwa

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Tags: Jim Dunwell, Andy Wetten, Transgenic Plants, Methods and Protocols