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Vaccinia Virus and Poxvirology Methods and Protocols 1st Edition by Stuart Isaacs 1588292290 9781588292292

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Vaccinia Virus and Poxvirology Methods and Protocols 1st Edition Stuart N. Isaacs (Auth.) Digital Instant Download

Author(s): Stuart N. Isaacs (auth.), Stuart N. Isaacs (eds.)
ISBN(s): 9781592597895, 1592597890
Edition: 1
File Details: PDF, 8.15 MB
Year: 2004
Language: english
SKU: EB-1367456 Category: Tags: ,

Vaccinia Virus and Poxvirology Methods and Protocols 1st Edition by Stuart N. Isaacs – Ebook PDF Instant Download/Delivery: 1588292290, 978-1588292292
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Product details:

ISBN 10:         1588292290
ISBN 13: 978-1588292292
Author:          Stuart N. Isaacs

A comprehensive collection of cutting-edge methods to study and work with the vaccinia virus and other poxvirus gene transcriptions. These readily reproducible techniques can be used for the construction and characterization of recombinant viruses; for the study of poxvirus gene transcription and DNA replication; for the investigation of the binding, entry, and movement of the virus in host cells; and for the use of the virus in immunologic and cell-cell fusion assays. Other methods cover poxvirus bioinformatics, aspects of viral pathogenesis at both a protein and an animal model level, and the study of immune responses to poxviruses-the latter a critical ability given the important role of vaccinia virus in smallpox vaccination and their potential role as vaccine vectors directed against infectious agents and cancer.
 

Vaccinia Virus and Poxvirology Methods and Protocols 1st Table of contents:

Part I: Introduction to Poxviruses

  1. Poxviruses: An Overview
    • 1.1 General Characteristics of Poxviruses
    • 1.2 Vaccinia Virus and Its Applications
    • 1.3 Poxvirus Replication Cycle
    • 1.4 The Role of Poxviruses in Gene Therapy

Part II: Molecular and Cellular Techniques
2. Molecular Cloning of Poxviruses

  • 2.1 Construction of Recombinant Vaccinia Viruses
  • 2.2 Insertion and Deletion of Genes in Poxviruses
  • 2.3 Plasmid-based Cloning Techniques
  • 2.4 Use of Selectable Markers

  1. Poxvirus Gene Expression Systems

    • 3.1 Transfection and Infection Techniques
    • 3.2 Optimizing Gene Expression in Poxviruses
    • 3.3 Inducible Systems for Poxvirus Gene Expression

  2. Virus Titration and Plaque Assays

    • 4.1 Basic Virus Quantification Methods
    • 4.2 Plaque Assays for Vaccinia Virus
    • 4.3 Determining Viral Yield and Infectivity

  3. Western Blotting and Immunofluorescence in Poxvirus Research

    • 5.1 Protein Detection Using Western Blotting
    • 5.2 Immunofluorescence Microscopy for Poxvirus Detection
    • 5.3 Antibody Generation and Immunological Assays

Part III: Poxvirus Pathogenesis and Immunology
6. Poxvirus Infection Models

  • 6.1 Animal Models for Vaccinia Virus Infection
  • 6.2 In Vivo Studies of Poxvirus Pathogenesis
  • 6.3 Mouse Models of Poxvirus Disease

  1. Immune Response to Vaccinia Virus

    • 7.1 Overview of Host Immune Response to Vaccinia
    • 7.2 Cell-Mediated Immunity and Vaccinia Virus
    • 7.3 Humoral Immunity and Antibodies in Poxvirus Infections

  2. Vaccinia Virus and Vaccine Development

    • 8.1 Role of Vaccinia Virus in Smallpox Eradication
    • 8.2 Vaccinia Virus as a Vaccine Vector
    • 8.3 Development of Novel Vaccinia Virus-Based Vaccines

Part IV: Advanced Techniques in Poxvirus Research
9. Reverse Genetics of Vaccinia Virus

  • 9.1 Generation of Mutant Vaccinia Viruses
  • 9.2 Use of Recombinant DNA Technology in Poxvirus Studies
  • 9.3 The Role of Reverse Genetics in Poxvirus Pathogenesis

  1. Live Imaging of Poxvirus Infections

    • 10.1 Techniques for Real-time Visualization of Virus Infections
    • 10.2 Live Cell Imaging in Poxvirus Research
    • 10.3 Fluorescence Microscopy in Virus Tracking

  2. Poxvirus Vectors in Gene Therapy

    • 11.1 Poxviruses as Vectors for Gene Delivery
    • 11.2 Gene Therapy Applications and Clinical Trials
    • 11.3 Safety Considerations and Regulatory Issues

Part V: Poxvirus Systems in Biotechnology
12. Poxviruses in Protein Production
– 12.1 Using Vaccinia Virus as a Protein Expression System
– 12.2 Methods for High-Scale Protein Production
– 12.3 Applications in Biopharmaceuticals

  1. Poxvirus-Based Nanotechnologies
    • 13.1 Poxvirus Particles in Nanomedicine
    • 13.2 Applications in Drug Delivery Systems
    • 13.3 Nanotechnology Approaches for Poxvirus Research

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