Molecular Epidemiology of Microorganisms Methods and Protocols 1st Edition by Michel Tibayrenc,Dominique Caugant ISBN 9781603279987 1603279989

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ISBN 10: 1603279989

ISBN 13:  9781603279987

Author: Michel Tibayrenc,Dominique A. Caugant 

The development and rapid implementation of molecular genotyping methods have re- lutionized the possibility for differentiation and classification of microorganisms at the subspecies level. Investigation of the species diversity is required to determine molecular relatedness of isolates for epidemiological studies. Methods for molecular epidemiology of microorganisms must be highly reproducible and provide effective discrimination of epidemiologically unrelated strains. A wide range of techniques has been applied to the investigation of outbreaks of tra- missible disease, and these have been critical in unraveling the route of spread of pat- gens for humans, animals, and plants. The choice of a molecular method will depend on the type of questions to be addressed, on the degree of genetic diversity of the species to be analyzed, and on the mechanisms responsible for generation of the diversity. The applications of molecular methods, singly or in combination, have greatly contributed in the past two decades to basic microbial science and public health control strategies. Molecular Epidemiology of Microorganisms: Methods and Protocols brings together a series of methods-based chapters with examples of application to some of the most important microbes. Both traditional and novel techniques are described, and the type of information that can be expected to be obtained by their application is indicated.

Table of contents: 

1. Microbial Molecular Epidemiology: An Overview – Michel Tibayrenc

2. Multilocus Enzyme Electrophoresis for Parasites and Other Pathogens – Michel Tibayrenc

3. Plasmid Replicon Typing – Timothy J. Johnson, Lisa K. Nolan

4. The Application of Randomly Amplified DNA Analysis in the Molecular Epidemiology of Microorganisms – Alex van Belkum, Elisabeth van Pelt-Verkuil, John P. Hays

5. Use of Repetitive Element Palindromic PCR (rep-PCR) for the Epidemiologic Discrimination of Foodborne Pathogens – Kelli L. Hiett, Bruce S. Seal

6. Pulsed-Field Gel Electrophoresis for Molecular Epidemiology of Food Pathogens – Tansy M. Peters

7. Molecular Genotyping of Microbes by Multilocus PCR and Mass Spectrometry: A New Tool for Hospital Infection Control and Public Health Surveillance – David J. Ecker, Christian Massire, Lawrence B. Blyn, Steven A. Hofstadler, James C. Hannis, Mark W. Eshoo et al.

8. Amplified Fragment Length Polymorphism Analysis – Norman K. Fry, Paul H.M. Savelkoul, Paolo Visca

9. Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) for the Genotyping of Bacterial Pathogens – Ibtissem Grissa, Gilles Vergnaud, Christine Pourcel

10. Spoligotyping for Molecular Epidemiology of the Mycobacterium tuberculosis Complex – Jeffrey R. Driscoll

11. Multilocus Sequence Typing – Ana Belén Ibarz Pavón, Martin C.J. Maiden

12. Multiple Locus Variable Number of Tandem Repeats Analysis – Gilles Vergnaud, Christine Pourcel

13. Comparison of Molecular Typing Methods Applied to Clostridium difficile – Ed J. Kuijper, Renate J. van den Berg, Jon S. Brazier

14. Genotyping of Mycobacterium tuberculosis Clinical Isolates Using IS6110-Based Restriction Fragment Length Polymorphism Analysis – Pablo Bifani, Natalia Kurepina, Barun Mathema, Xiao-Ming Wang, Barry Kreiswirth

15. spa Typing for Epidemiological Surveillance of Staphylococcus aureus – Marie Hallin, Alexander W. Friedrich, Marc J. Struelens

16. Sequencing of Viral Genes – Carol Holm-Hansen, Kirsti Vainio

17. Full Sequencing of Viral Genomes: Practical Strategies Used for the Amplification and Characterization of Foot-and-Mouth Disease Virus – Eleanor M. Cottam, Jemma Wadsworth, Nick J. Knowles, Donald P. King

18. Bacterial Genome Sequencing – Hervé Tettelin, Tamara Feldblyum

19. DNA Microarray for Molecular Epidemiology of Salmonella

1. Methods for Data Analysis – William Paul Hanage, David Michael Aanensen

2. Internet-Based Sequence-Typing Databases for Bacterial Molecular Epidemiology – Keith A. Jolley

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Tags: Michel Tibayrenc, Dominique Caugant, Molecular Epidemiology, Microorganisms Methods