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PCR Detection of Microbial Pathogens Methods in Molecular Biology Vol 216 1st Edition by Konrad Sachse ISBN 1588290492 978-1588290496

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PCR Detection of Microbial Pathogens Methods in Molecular Biology Vol 216 1st Edition Konrad Sachse (Editor) Digital Instant Download

Author(s): Konrad Sachse (Editor), Joachim Frey (Editor)
ISBN(s): 9781592593446, 1592593445
Edition: 1
File Details: PDF, 4.63 MB
Year: 2002
Language: english
SKU: EB-2030128 Category: Tags: ,

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Product details: 

ISBN 10: 1588290492 

ISBN 13: 978-1588290496

Author: Konrad Sachse

Hands-on laboratory experts present a set of “classic” PCR-based methods for the identification and detection of important animal and food microbial pathogens, including several zoonotic agents. These proven techniques can be precisely applied to a wide variety of microbes, among them Campylobacter spp., chlamydiae, toxigenic clostridia, Escherichia coli (STEC), Listeria monocytogenes, mycoplasmas, salmonellae, and Yersinia enterocolitica. Additional chapters review the specificity and performance of diagnostic PCR analysis, the pre-PCR processing of samples, the critical aspects of standardizing PCR methods, and the general issues involved in using PCR technology for microbial diagnosis.
 

Table of contents: 

1 Specificity and Performance of Diagnostic PCR Assays – Konrad Sachse
2 Pre-PCR Processing of Samples – Peter Rådström, Rickard Knutsson, Petra Wolffs, Maria Dahlenborg, Charlotta Löfström
3 Critical Aspects of Standardization of PCR – Jeffrey Hoorfar, Nigel Cook
4 PCR Technology and Applications to Zoonotic Food-Borne Bacterial Pathogens – Peter Stephensen Lübeck, Jeffrey Hoorfar
5 Protocols – Front Matter
6 Detection, Identification, and Subtyping of Actinobacillus pleuropneumoniae – Joachim Frey
7 Identification and Differentiation of Brucella abortus Field and Vaccine Strains by BaSS-PCR – Darla R. Ewalt, Betsy J. Bricker
8 Isolation of Campylobacter and Identification by PCR – Mark D. Englen, Scott R. Ladely, Paula J. Fedorka-Cray
9 Detection and Differentiation of Chlamydiae by Nested PCR – Konrad Sachse, Helmut Hotzel
10 Detection of Toxigenic Clostridia – Michel R. Popoff
11 PCR-Based Detection of Coxiella burnetii from Clinical Samples – Mustapha Berri, Nathalie Arricau-Bouvery, Annie Rodolakis
12 Detection and Subtyping of Shiga Toxin-Producing Escherichia coli (STEC) – Peter Gallien
13 Detection of Listeria monocytogenes Using a PCR/DNA Probe Assay – Louise O’Connor
14 Detection of Leptospira interrogans – John W. Lester, Rance B. LeFebvre
15 Detection of Pathogenic Mycobacteria of Veterinary Importance – Robin A. Skuce, M. Siobhan Hughes, Malcolm J. Taylor, Sydney D. Neill
16 Multiplex PCR of Avian Pathogenic Mycoplasmas – Mazhar I. Khan
17 Detection and Differentiation of Ruminant Mycoplasmas – Helmut Hotzel, Joachim Frey, John Bashiruddin, Konrad Sachse
18 Detection of Mycoplasma hyopneumoniae from Clinical Samples and Air

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Tags: Konrad Sachse, PCR Detection, Microbial Pathogens, Molecular Biology