Applied Thin Layer Chromatography Best Practice and Avoidance of Mistakes 2nd Edition by Hahn Deinstrop Elke ISBN 3527315536 978-3527315536

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ISBN 10: 3527315536

ISBN 13:  978-3527315536

Author: Hahn-Deinstrop Elke

Thin-layer chromatography (TLC) is a powerful, fast and inexpensive analytical method. It has proven its usefulness in pharmaceutical, food and environmental analysis. This new edition of the practical TLC guide features a completely revised chapter on documentation, now including the use of digital cameras. Selected new sorbents and instruments are also introduced.

Why has the prior edition been successful? All steps of the analytical procedure are clearly explained, starting with the choice of a suitable TLC technique and ending with data evaluation and documentation. Special emphasis is put on the proper choice of materials for TLC. Properties and functions of various materials and the TLC equipment are described, covering e.g. precoated layers, solvents and developing chambers, including information on suppliers. Many practical hints for trouble shooting are given. All this is illustrated with numerous coloured figures.

How to use TLC in compliance with GLP/GMP regulations is described in detail, including the required documentation. Therefore the reader can very easily compile his own standard operating procedures.

Table of contents: 

1. Introduction

2. What Does TLC Mean?

3. When Is TLC Used?

4. Where Is TLC Used?

5. How Is the Result of a TLC Represented?

6. What Kinds of Reference Substances Are Used in TLC?

7. The Literature on TLC

8. Precoated Layers

9. Precoated Layers—Why?

10. How Are Precoated Layers Produced?

11. What Types of Precoated Layers Are There?

12. What Are the Uses of Precoated Layers?

13. Criteria for the Selection of Stationary Phases in TLC

14. Effect of the Stationary Phase When Mobile Phases Are Identical

15. Advice on the Ordering and Storage of Precoated Layers

16. Problems in the Naming and Arrangement of Precoated Layers

17. Before the TLC Development Process

18. Handling of Precoated Layers

19. Prewashing

20. Activation

21. Conditioning

22. Impregnation

23. Application of Samples

24. Positioning of the Samples

25. Drying Before the Development

26. Solvent Systems, Developing Chambers and Development

27. Solvent Systems

28. TLC Developing Chambers

29. Development of Thin-Layer Chromatograms

30. Drying After Development

31. Evaluation Without Derivatization

32. Direct Visual Evaluation

33. Direct Optical Evaluation Using Instruments

34. Diode-Array Detection

35. Coupled Methods for Substance Identification

36. Documentation Without or Before Derivatization

37. Derivatization

38. Thermochemical Reaction

39. Irradiation with High-Energy Light

40. Reaction with Reagents

41. Special Cases of Derivatization

42. Further Treatment of Derivatized Chromatograms

43. Evaluation After Derivatization

44. Visual Evaluation

45. Evaluation Using a TLC Scanner

46. Evaluation Using a Video System

47. Evaluation by Flat-Bed Scanner

48. Evaluation Using a Digital Camera

49. Documentation

50. Description of a Thin-Layer Chromatogram

51. Documentation by Drawing, Tracing and Photocopying

52. Photographic Documentation

53. Video Documentation

54. Documentation With Digital Cameras

55. TLC Scanner Documentation

56. Flat-Bed Scanner Documentation

57. Bioluminescence Measurements

58. GMP/GLP-Conforming Operations in TLC

59. Validation of TLC Methods

60. Use of Qualified/Calibrated Equipment

61. GMP/GLP-Conforming Raw Data Sheets

62. Examples of GMP/GLP-Conforming Testing Procedures (TPs)

63. Effects of Stress

64. Controlled Stress on a Substance

65. TLC-Sensitive Substances

66. Special Methods in TLC

67. AMD—Automated Multiple Development

68. OPLC—Overpressured Layer Chromatography

69. HPPLC—High Pressure Planar Liquid Chromatography

70. TLC-FID/FTID—Combination of TLC and Flame-Ionization Detector or Flame-Thermionic Ionization Detector

71. TLC-NDIR

72. RPC—Rotation Planar Chromatography

73. Appendix

74. CHROMart

75. References

76. Abbreviations

77. Acknowledgements

78. Market Overview

79. Photograph Section

80. Subject Index

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Tags: Hahn Deinstrop Elke, Applied Thin Layer, Chromatography Best Practice, Avoidance of Mistakes