Xenopus Protocols Post Genomic Approaches 2nd Edition by STEFAN HOPPLER, Peter D Vize ISBN 9781617799921 1617799920

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Product details: 

ISBN 10:1617799920

ISBN 13:  9781617799921

Author: STEFAN HOPPLER, Peter D Vize

During the 20th century Xenopus has flourished as an accessible experimental model system for studying vertebrate biology. The second edition of  Xenopus Protocols:Post-Genomic Approaches expands upon the previous edition with novel approaches inspired by Xenopus tropicalis and the sequencing of its genome. Xenopus tropicalis genomics and tractable genetics combined with the unique experimental advantages of Xenopus laevis and their distinctive phylogenetic position make them unique model systems for vertebrate embryonic development in the 21st century. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Xenopus Protocols: Post-Genomic Approaches, Second Edition provides a toolkit that will enable every laboratory to maximize the power of this extraordinary experimental system in the post-genomic age.  

Table of contents: 

1. Front Matter

2. Xenopus tropicalis

3. Xenopus tropicalis as a Model Organism for Genetics and Genomics: Past, Present, and Future by Robert M. Grainger

4. Husbandry of Xenopus tropicalis by Alan Jafkins, Anita Abu-Daya, Anna Noble, Lyle B. Zimmerman, Matthew Guille

5. Generating Diploid Embryos from Xenopus tropicalis by Florencia del Viso, Mustafa Khokha

6. Navigating the Xenopus tropicalis Genome by Ira L. Blitz

7. Genetics in Xenopus

8. Genetic Analysis of Xenopus tropicalis by Timothy J. Geach, Derek L. Stemple, Lyle B. Zimmerman

9. Forward Genetic Screens in Xenopus Using Transposon-Mediated Insertional Mutagenesis by Donald A. Yergeau, Clair M. Kelley, Haiqing Zhu, Emin Kuliyev, Paul E. Mead

10. Targeted Gene Disruption with Engineered Zinc-Finger Nucleases (ZFNs) by John J. Young, Richard M. Harland

11. Reverse Genetic Studies Using Antisense Morpholino Oligonucleotides by Yanan Zhao, Shoko Ishibashi, Enrique Amaya

12. Chemical Genetics and Drug Discovery in Xenopus by Matthew L. Tomlinson, Adam E. Hendry, Grant N. Wheeler

13. Maternal mRNA Knock-down Studies: Antisense Experiments Using the Host-Transfer Technique in Xenopus laevis and Xenopus tropicalis by David J. Olson, Alissa M. Hulstrand, Douglas W. Houston

14. Xenopus Transgenesis

15. Generating Transgenic Frog Embryos by Restriction Enzyme Mediated Integration (REMI) by Shoko Ishibashi, Kristen L. Kroll, Enrique Amaya

16. A Simple Method of Transgenesis Using I-Sce I Meganuclease in Xenopus by Shoko Ishibashi, Nick R. Love, Enrique Amaya

17. Using ΦC31 Integrase to Mediate Insertion of DNA in Xenopus Embryos by You E. Li, Bryan G. Allen, Daniel L. Weeks

18. Xenopus Transgenics: Methods Using Transposons by Clair M. Kelley, Donald A. Yergeau, Haiqing Zhu, Emin Kuliyev, Paul E. Mead

19. Comparative Genomics-Based Identification and Analysis of Cis-Regulatory Elements by Hajime Ogino, Haruki Ochi, Chihiro Uchiyama, Sarah Louie, Robert M. Grainger

20. Tet-On Binary Systems for Tissue-Specific and Inducible Transgene Expression

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