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(Ebook PDF) Nitrogen Fixation Methods and Protocols 1st Edition by Markus Ribbe 1617791949 9781617791949 Full chapter

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Nitrogen Fixation Methods and Protocols 1st Edition Yilin Hu Digital Instant Download

Author(s): Yilin Hu, Markus W. Ribbe (auth.), Markus W. Ribbe (eds.)
ISBN(s): 9781617791949, 1617791946
Edition: 1
File Details: PDF, 6.09 MB
Year: 2011
Language: english
SKU: EB-2451688 Category: Tags: , ,

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Product details:

ISBN 10:         1617791949
ISBN 13: 978-1617791949
Author:          Markus W. Ribbe

Few problems in protein biochemistry have proven to be as challenging and recalcitrant as the molecular description of nitrogenase, the catalyst of one of the most remarkable chemical transformations in biological systems: the nucleotide-dependent reduction of atmospheric dinitrogen to bioavailable ammonia.  In Nitrogen Fixation: Methods and Protocols, recognized experts in the field provide an up-to-date, in-depth overview of the methods that have been applied to studying the nitrogenase at a molecular level, ranging from genetic, biochemical, spectroscopic, and chemical methods to theoretical calculations. In addition, techniques used to study an enzyme system that is homologous to nitrogenase are described in this book.  Written in the highly successful Methods in Molecular Biology™ series format, methods chapters include introductions to their respective chapters, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Practical and cutting-edge, Nitrogen Fixation: Methods and Protocols will be useful for anyone interested in nitrogenase research and willing to venture further toward addressing the remaining mechanistic and biosynthetic questions of this fascinating enzyme system.

Nitrogen Fixation Methods and Protocols 1st Table of contents:

  1. Historic Overview of Nitrogenase Research

    • Yilin Hu, Markus W. Ribbe

  2. Mechanism of Mo-Dependent Nitrogenase

    • Zhi-Yong Yang, Karamatullah Danyal, Lance C. Seefeldt

  3. Assembly of Nitrogenase MoFe Protein

    • Chi-Chung Lee, Aaron W. Fay, Jared A. Wiig, Markus W. Ribbe, Yilin Hu

  4. Genomic Analysis of Nitrogen Fixation

    • Ina P. O’Carroll, Patricia C. Dos Santos

  5. Enzymatic Systems with Homology to Nitrogenase

    • Jürgen Moser, Markus J. Bröcker

  6. Molecular Biology and Genetic Engineering in Nitrogen Fixation

    • Patricia C. Dos Santos

  7. Purification of Nitrogenase Proteins

    • Jared A. Wiig, Chi-Chung Lee, Aaron W. Fay, Yilin Hu, Markus W. Ribbe

  8. Assays of Nitrogenase Reaction Products

    • William E. Newton, Michael J. Dilworth

  9. Methods for Nitrogenase-Like Dark Operative Protochlorophyllide Oxidoreductase

    • Jürgen Moser, Markus J. Bröcker

  10. X-Ray Crystallography

    • Lauren E. Roth, F. Akif Tezcan

  11. X-Ray Absorption Spectroscopy

    • Serena DeBeer

  12. Small Angle X-Ray Scattering Spectroscopy

    • David W. Mulder, John W. Peters

  13. Electron Paramagnetic Resonance Spectroscopy

    • Karamatullah Danyal, Zhi-Yong Yang, Lance C. Seefeldt

  14. Magnetic Circular Dichroism Spectroscopy

    • Brian J. Hales

  15. Mössbauer Spectroscopy

  16.  

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