Proteomics in Practice A Guide to Successful Experimental Design Second Edition by Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker – Ebook PDF Instant Download/Delivery: 978-3527319411, 3527319411
Full download Proteomics in Practice A Guide to Successful Experimental Design Second Edition after payment

Product details:
ISBN 10: 3527319411
ISBN 13: 978-3527319411
Author: Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker
Still the only concise practical guide to laboratory experiments in proteomics, this new edition now also covers DIGE technology and liquid-chromatography, while the troubleshooting section has been considerably extended.
Adopting a practical approach, the authors present the relevant techniques and explain the route to successful experimental design and optimal method selection. They cover such electrophoretic techniques as isoelectric focusing, SDS page, 2-D page, and DIGE, as well as liquid-chromatography techniques, such as ion exchange, affinity chromatography and reversed-phase HPLC. Mass-spectrometric techniques include MALDI, ESI, and FT ICR.
Generously illustrated, partly in color, the book also features updates of protocols as well as animations illustrating crucial methodological steps on a companion website.
Table of contents:
INTRODUCTION
History
Critical Points
Hypothesis Driven Approach vs. High Throughput
Complementarities of Workflows
Concept of Experimental Planning
Success Stories, Highlights of Useful References
PART I: PROTEOMICS TECHNOLOGY
Electrophoresis
Liquid Chromatography
Mass Spectrometry
Bioinformatics Tools / Data Mining
Methods Under Development
PART II: PRACTICAL MANUAL OF PROTEOME ANALYSIS
Strategy Where to Start
Gel-Based Workflow
LC-Based Workflow
Latest Trends
Validation with Western Blotting
Statistic Evaluation
PART III: TROUBLE SHOOTING
Gel-Based Workflow
LC-Based Workflow
People also search for:
proteomics in clinical trials
proteomics examples
proteomics protocol
proteomics is the study of
bioinformatics proteomics
Tags: Reiner Westermeier, Tom Naven, Hans-Rudolf Höpker, A Guide, Experimental Design


