Biotechnology and Genetics in Fisheries and Aquaculture 1st Edition by Andy Beaumont, Hoare ISBN 978-0632055159 0632055154
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Product details:
ISBN 10: 0632055154
ISBN 13: 978-0632055159
Author: Andy Beaumont, K. Hoare
The recent development of molecular biology and genetic techniques, in particular those that are increasingly being used in practical situations in fish biology, fisheries and aquaculture, has led to a gap in the understanding by many of the science behind these techniques and their correct implementation for maximum results.
The authors of this important book, Andy Beaumont and Kate Hoare, have written a text of great clarity, which carefully explains the science and application of molecular and genetic techniques to fisheries and aquaculture situations and what these new technologies have to offer. Contents include a full explanation of genetic variation and its measurement, genetic structure in natural populations, genetics and artificial selection in the hatchery, ploidy manipulation and the use of genetic engineering in aquaculture.
Biotechnology and Genetics in Fisheries and Aquaculture is of great use to biological sciences students, particularly those studying marine, freshwater and aquatic biology, fish biology, fisheries, aquaculture, population biology and genetics. The book is also extremely useful as a reference to personnel such as fish farmers and fisheries scientists and all those working in fisheries and aquaculture management and research. Libraries in all universities and research establishments where biological sciences, fisheries and aquaculture are studied and taught should have copies of this book on their shelves.
Table of contents:
1 What is Genetic Variation?
Deoxyribose nucleic acid: DNA
Ribose nucleic acid: RNA
What is the genetic code?
Protein structure
So what about chromosomes?
How does sexual reproduction produce variation?
Mitochondrial DNA
Further reading
2 How Can Genetic Variation be Measured?
DNA sequence variation
DNA fragment size variation
Restriction fragment length polymorphisms (RFLPs)
Variable number tandem repeats (VNTR)
DNA fingerprinting
Random amplified polymorphic DNA (RAPD)
Amplified fragment length polymorphism (AFLP)
Protein variation
Phenotypic variation
Further reading
3 Genetic Structure in Natural Populations
What is a stock?
How are allele frequencies estimated?
What is the relationship between alleles and genotypes?
How do allele frequencies change over time?
How does population structure arise?
How are genetic markers used to define population structure?
Levels of genetic differentiation in aquatic organisms
Mixed stock analysis (MSA)
Conservation genetics
Further reading
4 Genetic Considerations in the Hatchery
Is there evidence of loss of genetic variation in the hatchery?
How do hatcheries affect heterozygosity?
How can we use genetic markers to identify hatchery-produced individuals?
Identification to family level
Identification to population level
Genome mapping
How is a genome mapped?
How do we carry out linkage analysis?
The SALMAP project
Identification of diseases
Further reading
5 Artificial Selection in the Hatchery
Qualitative traits
Quantitative traits
What kinds of traits are important?
Variance of a trait
How can we estimate narrow-sense heritability?
Correlated traits
What types of artificial selection are there?
What about realised heritabilities?
Setting up a breeding programme
Inbreeding, cross-breeding and hybridisation
Further reading
6 Triploids and Beyond: Why Manipulate Ploidy?
How is it done?
Production of gynogens and androgens
Identification of ploidy change
Triploids
Tetraploids
Gynogens and androgens
Further reading
7 Genetic Engineering in Aquaculture
The DNA construct
The transgene
The promoter
Transgene delivery
Microinjection
Electroporation
Sperm-mediated transfer
Biolistics
Viral vectors
Lipofection
Transgene integration
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Tags: Andy Beaumont, Hoare, Biotechnology and Genetics, Fisheries and Aquaculture