Signal Transduction Immunohistochemistry Methods and Protocols 1st Edition by Kathy Brumbaugh ISBN 9781617790249, 1617790249

Signal Transduction Immunohistochemistry Methods and Protocols 1st Edition by Kathy Brumbaugh  – Ebook PDF Instant Download/Delivery:  9781617790249, 1617790249
Full download Signal Transduction Immunohistochemistry Methods and Protocols 1st Edition  after payment

Product details: 

ISBN 10:  1617790249

ISBN 13: 9781617790249

Author: Kathy Brumbaugh

Unlike detecting constitutively expressed targets, immunohistochemical detection of labile, low abundance, and short-lived signal transduction molecules can be a very difficult task.  In Signal Transduction Immunohistochemistry: Methods and Protocols, IHC experts contribute detailed protocols addressing the numerous challenges of signal-transduction immunohistochemistry (ST-IHC).  Beginning with a set of introductory chapters, the volume moves on to cover techniques used for the preservation of antigens and their unmasking, protocols in digital imaging and image analysis of stained cells and tissues, high-throughput data collection and data analysis, and techniques used in neuroscience as well as cancer and stem cell research.  Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Signal Transduction Immunohistochemistry: Methods and Protocols serves as an ideal guide for novices and as a bastion of inspiring ideas to be exploited by experienced researchers on the lookout for new experimental tricks and hints.

Table of contents: 

Antibodies as a Tool: From Concept to Design and Application

1. Overview of the Generation, Validation, and Application of Phosphosite-Specific Antibodies – Kathy Brumbaugh, Wade Johnson, Wen-Chieh Liao, Mong-Shang Lin, J. P. Houchins, Jeff Cooper et al.

2. Selection and Validation of Antibodies for Signal Transduction Immunohistochemistry – Juraj Bodo, Eric D. Hsi

3. An Overview of Western Blotting for Determining Antibody Specificities for Immunohistochemistry – Biji T. Kurien, Yaser Dorri, Skyler Dillon, Anil Dsouza, R. Hal Scofield

4. Optimized Protocol to Make Phospho-Specific Antibodies that Work – Amy J. Archuleta, Crystal A. Stutzke, Kristin M. Nixon, Michael D. Browning

Preservation and Unmasking of Tissue Antigens
5. Methodology and Technology for Stabilization of Specific States of Signal Transduction Proteins – Mats Borén
6. An Enhanced Antigen-Retrieval Protocol for Immunohistochemical Staining of Formalin-Fixed, Paraffin-Embedded Tissues – Sergei I. Syrbu, Michael B. Cohen

Imaging Techniques and High-Throughput Data Analysis
7. Imaging Techniques in Signal Transduction IHC – Jerry Sedgewick
8. Practical Considerations of Image Analysis and Quantification of Signal Transduction IHC Staining – Michael Grunkin, Jakob Raundahl, Niels T. Foged
9. Flow Cytometric Analysis of Cell Signaling Proteins – Maria A. Suni, Vernon C. Maino
10. CytoSys: A Tool for Extracting Cell-Cycle-Related Expression Dynamics from Static Data – Jayant Avva, Michael C. Weis, Radina P. Soebiyanto, James W. Jacobberger, Sree N. Sreenath

Neuroscience, Cancer, and Stem Cell Research
11. Signaling Events Initiated by Kappa Opioid Receptor Activation: Quantification and Immunocolocalization Using Phospho-Selective KOR, p38 MAPK, and KIR 3.1 Antibodies – Julia C. Lemos, Clarisse A. Roth, Charles Chavkin
12. Immunohistochemical Assessment of Signal Transduction and Cell-Cycle Networks in Neural Tumors – Daniel Ciznadija, Afsar Barlas, Katia Manova
13. Novel Multicolor Immunofluorescence Technique Using Primary Antibodies Raised in the Same Host Species – Jillian Frisch, J. P. Houchins, Michael Grahek, Jordan Schoephoerster, Jodi Hagen, Joseph Sweet et al.
14. Activation and Differentiation of Mesenchymal Stem Cells – Pravin J. Mishra, Debabrata Banerjee

Novel Assays and Techniques
15. Double In Situ Detection of Sonic Hedgehog mRNA and pMAPK Protein in Examining the Cell Proliferation Signaling Pathway in Mouse Embryo – Sho Fujisawa, Mesruh Turkekul, Afsar Barlas, Ning Fan, Katia Manova
16. Identifying Intracellular Sites of Eicosanoid Lipid Mediator Synthesis with EicosaCell Assays – Christianne Bandeira-Melo, Peter F. Weller, Patricia T. Bozza
17. Absorption Control in Immunohistochemistry Using Phospho-Peptides Immobilized on Magnetic Beads

People also search for:

a signal transduction pathway
    
a signal transduction cascade
    
signal transduction in b cells
    
cell signal transduction pathway in the immune system
    
signal transduction in t cells

Tags: Kathy Brumbaugh, Signal Transduction, Immunohistochemistry