Regulated cell death Part A apoptotic mechanisms 1st Edition by Avi Ashkenazi, Junying Yuan, Jim Wells ISBN 0124171583 978-0124171589

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Product details: 

ISBN 10:  0124171583 

ISBN 13: 978-0124171589

Author: Avi Ashkenazi,  Junying Yuan, Jim Wells

Regulated Cell Death Part A & Part B of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in apoptosis focusing on the important areas of intrinsic pathway, extrinsic pathway, caspases, cellular assays and post-apoptotic effects and model organisms; as well as topics on necroptosis and screening approaches.
Continues the legacy of this premier serial with quality chapters authored by leaders in the field
Covers research methods in biomineralization science
Regulated Cell Death Part A & Part B contains sections on such topics as apoptosis focusing on the important areas of intrinsic pathway, extrinsic pathway, caspases, cellular assays and post-apoptotic effects and model organisms; as well as topics on necroptosis and screening approaches

Table of contents: 

1 Introduction
2 An In Vitro Fluorescence-Based Liposome System
2.1 Expression and purification of Bcl-2 family proteins
2.2 Site-specific protein labeling
2.3 Production of mitochondria-like liposomes
3 Membrane Permeabilization Assay
3.1 ANTS/DPX release assay
4 Fluorescence Resonance Energy Transfer
4.1 Detecting the interaction between two proteins using FRET
5 Tracking the Conformation Changes of a Protein
5.1 NBD-emission assay
6 Determining the Topology of Proteins within Membranes
6.1 Iodide quenching of NBD-labeled Bax
7 Conclusion

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Avi Ashkenazi,Junying Yuan,Jim Wells,Regulated cell,Part A apoptotic