Olfactory Receptors Methods and Protocols 1st Edition by Chiquito Crasto ISBN 978-1627033770 1627033770

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Product details: 

ISBN 10:  1627033770 

ISBN 13: 978-1627033770

Author: Chiquito J. Crasto

Despite the best efforts of many and despite landmark discoveries and experimental ingenuity, challenges in the pursuit of research related to olfactory receptors (ORs) continue to exist.  In Olfactory Receptors: Methods and Protocols, experts in the field contribute chapters that serve to address these challenges.  The volume consists of several sections: knowledge dissemination of ORs, theoretical assessments of OR structure and function, as well as development and use of expression systems and experimental functional analysis. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Concise and practical,  Olfactory Receptors: Methods and Protocols seeks to aid researchers in furthering the knowledge of olfaction and moving us ever closer to the thrilling discoveries that will follow.

Table of contents: 

Knowledge Dissemination of Olfactory Receptors

1 Preface
2 Contributors to the First Edition
3 Contributors to the Second Edition
4 Abbreviations
5 Conversion Factors
6 Classification of Cases
7 Questions
8 Answers
9 FAQs
10 References

Databases and Genomics of Olfactory Receptors

11 Databases in SenseLab for the Genomics, Proteomics, and Function of Olfactory Receptors – Luis N. Marenco, Gautam Bahl, Lorra Hyland, Jing Shi, Rixin Wang, Peter C. Lai et al.
12 HORDE: Comprehensive Resource for Olfactory Receptor Genomics – Tsviya Olender, Noam Nativ, Doron Lancet
13 Identification of Olfactory Receptor Genes from Mammalian Genome Sequences – Yoshihito Niimura

Theoretical Assessments of Olfactory Receptor Structure and Function

14 Molecular Modelling of Odorant/Olfactory Receptor Complexes – Landry Charlier, Jérémie Topin, Claire A. de March, Peter C. Lai, Chiquito J. Crasto, Jerome Golebiowski
15 The Electrical Properties of Olfactory Receptors in the Development of Biological Smell Sensors – Eleonora Alfinito, Jeremy Pousset, Lino Reggiani
16 A Hit Map-Based Statistical Method to Predict Best Ligands for Orphan Olfactory Receptors: Natural Key Odorants Versus “Lock Picks” – Dietmar Krautwurst, Matthias Kotthoff

Expression Systems for Olfactory Receptors

17 A Robust, Rapid, and Simple Method of Producing Olfactory Receptors Using Commercial E. coli Cell-Free Systems – Karolina Corin, Brian Cook, Shuguang Zhang
18 Human Olfactory Receptors: Recombinant Expression in the Baculovirus/Sf9 Insect Cell System, Functional Characterization, and Odorant Identification – Valéry Matarazzo, Catherine Ronin
19 Single Cell RT-PCR Identification of Odorant Receptors Expressed by Olfactory Neurons – Bettina Malnic
20 Next-Generation Sequencing of the Human Olfactory Receptors – Joel D. Mainland, Jason R. Willer, Hiroaki Matsunami, Nicholas Katsanis
21 Deciphering Activation of Olfactory Receptors Using Heterologous Expression in Saccharomyces cerevisiae and Bioluminescence Resonance Energy Transfer – Guenhaël Sanz, Edith Pajot-Augy
22 Olfactory Receptors in Human Airway Epithelia – Xiaoling Gu, Yehuda Ben-Shahar

Functional Analysis of Olfactory Receptors

23 Functional Characterization of Insect Olfactory Receptor Neurons Through In Vivo Approaches – Michel Renou, Philippe Lucas
24 Functional Assay of Mammalian and Insect Olfactory Receptors Using Xenopus Oocytes – Charles W. Luetje, Andrew S. Nichols, Ana Castro, Benjamin L. Sherman
25 Pharmacology of Mammalian Olfactory Receptors

26 Visualizing Olfactory Receptor Expression and Localization in Drosophila – Michael Saina, Richard Benton
27 Deorphanization of Human Olfactory Receptors by Luciferase and Ca-Imaging Methods – Kaveh A. Baghaei

Olfactory Receptor Evolution

28 Evolution of Olfactory Receptors

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Tags: Chiquito Crasto, Olfactory Receptors, Methods and Protocols