Allostery Methods and Protocols 1st Edition by James Kranz, José Clemente ISBN 9781627033381 1627033386

Allostery Methods and Protocols 1st Edition by James K. Kranz,  José C. Clemente- Ebook PDF Instant Download/Delivery: 9781627033381, 1627033386
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Product details: 

ISBN 10:1627033386

ISBN 13:  9781627033381

Author:  James K. Kranz,  José C. Clemente

Despite considerable variability within the scientific community, allosteric regulation can best be defined functionally as how a macromolecule binds one ligand differently when a second ligand is or is not pre-bound to the macromolecule, which constitutes a vital aspect of protein structure/function.  In Allostery: Methods and Protocols, expert researchers in the field provide key techniques to investigate this biological phenomenon.  Focusing on heterotropic systems with some coverage of homotropic systems, this volume covers the monitoring of allosteric function, allosteric conformational changes, and allosteric changes in protein dynamics/sub-population distribution, as well as topics such as macromolecular and ligand engineering of allosteric functions and computational aids in the study of allostery. Written in the highly successful Methods in Molecular Biology™ series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory.

 

Thorough and intuitive, Allostery: Methods and Protocols aids scientists in continuing to study ligand-induced, through-protein effects on protein function (ligand binding/catalysis), a phenomenon that is well recognized through the history of the life sciences and very poorly understood at the molecular level.

Table of contents: 

Monitoring Allosteric Function

1 Monitoring Allosteric Function

• Front Matter
• Binding Techniques to Study the Allosteric Energy Cycle – James K. Kranz, José C. Clemente
• Kinetic Trapping of a Key Hemoglobin Intermediate – Jo M. Holt, Gary K. Ackers
• Allosteric Coupling Between Transition Metal-Binding Sites in Homooligomeric Metal Sensor Proteins – Nicholas E. Grossoehme, David P. Giedroc
• Studying the Allosteric Energy Cycle by Isothermal Titration Calorimetry – Marta Martinez-Julvez, Olga Abian, Sonia Vega, Milagros Medina, Adrian Velazquez-Campoy
• Detecting “Silent” Allosteric Coupling – Harvey F. Fisher
• Using Mutant Cycle Analysis to Elucidate Long-Range Functional Coupling in Allosteric Receptors – Jai A. P. Shanata, Shawnalea J. Frazier, Henry A. Lester, Dennis A. Dougherty

2 Monitoring Allosteric Conformational Changes

• Front Matter
• A Review of Methods Used for Identifying Structural Changes in a Large Protein Complex – Owen W. Nadeau, Gerald M. Carlson
• Allosteric Mechanisms of G Protein-Coupled Receptor Signaling: A Structural Perspective – Tarjani M. Thaker, Ali I. Kaya, Anita M. Preininger, Heidi E. Hamm, T. M. Iverson
• Dynamic Light Scattering to Study Allosteric Regulation – Aaron L. Lucius, P. Keith Veronese, Ryan P. Stafford
• Dissecting the Linkage Between Transcription Factor Self-Assembly and Site-Specific DNA Binding: The Role of the Analytical Ultracentrifuge – Amie D. Moody, James P. Robblee, David L. Bain
• Fluorescence Correlation Spectroscopy and Allostery: The Case of GroEL – Gabriel A. Frank, Amnon Horovitz, Gilad Haran
• The Morpheein Model of Allostery: Evaluating Proteins as Potential Morpheeins – Eileen K. Jaffe, Sarah H. Lawrence

3 Monitoring Allosteric Changes in Protein Dynamics/Subpopulation Distribution

• Front Matter
• Combining NMR and Molecular Dynamics Studies for Insights into the Allostery of Small GTPase–Protein Interactions – Liqun Zhang, Sabine Bouguet-Bonnet, Matthias Buck
• Hydrogen–Deuterium Exchange Study of an Allosteric Energy Cycle – Dorothy Beckett
• Ensemble Properties of Network Rigidity Reveal Allosteric Mechanisms – Donald J. Jacobs, Dennis R. Livesay, James M. Mottonen, Oleg K. Vorov, Andrei Y. Istomin, Deeptak Verma

4 Macromolecular and Ligand Engineering Allosteric Functions

• An In Vivo Approach to Isolating Allosteric Pathways Using Hybrid Multimeric Proteins – Cuijuan Tie, Gregory D. Reinhart
• Mutations in the GABAA Receptor that Mimic the Allosteric Ligand Etomidate – Stuart A. Forman, Deirdre Stewart
• Allosteric Regulation of Human Liver Pyruvate Kinase by Peptides that Mimic the Phosphorylated/Dephosphorylated N-Terminus – Charulata B. Prasannan, Qingling Tang, Aron W. Fenton
• In Silico-Screening Approaches for Lead Generation: Identification of Novel Allosteric Modulators of Human-Erythrocyte Pyruvate Kinase – Ashutosh Tripathi, Martin K. Safo
• Identification of Allosteric-Activating Drug Leads for Human Liver Pyruvate Kinase – Aron W. Fenton

5 Computational Methods/Aids in the Study of Allostery

• Front Matter
• A Critical Evaluation of Correlated Mutation Algorithms and Coevolution Within Allosteric Mechanisms – Dennis R. Livesay, Kyle E. Kreth, Anthony A. Fodor
• The Advantage of Global Fitting of Data Involving Complex Linked Reactions – Petr Herman, J. Ching Lee
• Predicting Binding Sites by Analyzing Allosteric Effects – Dengming Ming, Michael E. Wall

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